Ginkgolide J (BN 52024) has neuroprotective activity, it can prevent A beta(1-42) induced inhibition of long-term potentiation in the CA1 region of mouse hippocampal slices.

新型银杏叶提取物P8A（TTL），富含70%的萜烯三内酯，可防止Aβ抑制小鼠海马CA1区的长期增强效应。这种神经保护效果在很大程度上归功于Ginkgolide J，后者能完全复制提取物的效果。Ginkgolide J还能抑制由Aβ引起的啮齿类动物海马神经元的细胞死亡[1]。

Hippocampal cultures were treated by adding 10 μM Aβ1-42 in its oligomeric form with or without P8A, or alternatively with and without each of the individual ginkgolides and bilobalide (GA, GB, GC GJ, BB).??these compounds were dissolved in DMSO and added to the culture medium at a ratio of 1:1000 (v/v), yielding a 0.1% DMSO solution.?After 24 hrs the number of viable cells was assessed by nuclear counting.?In a separate set of experiments, viable cells were counted by using the ethidium homodimer/calcein-AM combination of vital dyes (LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells;?Invitrogen), according to the manufacturer instructions.?Values represent the mean SEM of three consecutive experiments.?Each experiment was performed in triplicate[1].